A yeast splicing factor is localized in discrete subnuclear domains.
AUTOR(ES)
Elliott, D J
RESUMO
Digital imaging microscopy has been used to visualize the splicing protein PRP6p and three other yeast nuclear proteins. The results show that PRP6p is uniquely localized to discrete subnuclear regions. A combination of cytological and biochemical assays suggests that these sites can be saturated when the protein is overexpressed and likely correspond to the location of U4/U6 snRNPs. The observations indicate that some splicing components are located in discrete subregions of the yeast nucleus, similar to the situation described for the mammalian nucleus.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=556833Documentos Relacionados
- Functional analysis of pre-mRNA splicing factor SF2/ASF structural domains.
- Nuclear factor I is specifically targeted to discrete subnuclear sites in adenovirus type 2-infected cells.
- Matrix attachment region binding protein MFP1 is localized in discrete domains at the nuclear envelope.
- Mitochondrial targeting of yeast apoiso-1-cytochrome c is mediated through functionally independent structural domains.
- Activation of polyomavirus DNA replication by yeast GAL4 is dependent on its transcriptional activation domains.
