Absence in Bacillus subtilis and Staphylococcus aureus of the sequence-specific deoxyribonucleic acid methylation that is conferred in Escherichia coli K-12 by the dam and dcm enzymes.
AUTOR(ES)
Dreiseikelmann, B
RESUMO
Restriction analysis of plasmid pHV14 deoxyribonucleic acid isolated from Escherichia coli K-12, Bacillus subtilis, and staphylococcus aureus with restriction endonucleases MboI, Sau3AI, and EcoRII was used to study the methylation of those nucleotide sequences which in E. coli contain the major portions of N6-methyladenine and 5-methylcytosine. The results showed that neither B. subtilis nor S. aureus methylates deoxyribonucleic acid at the same sites and nucleotides which are recognized and methylated by dam and dcm enzymes in E. coli K-12.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=216033Documentos Relacionados
- Sequence-specific and mechanism-based crosslinking of Dcm DNA cytosine-C5 methyltransferase of E. coli K-12 to synthetic oligonucleotides containing 5-fluoro-2'-deoxycytidine.
- Escherichia coli K-12 clones that overproduce dam methylase are hypermutable.
- Isolation of Deoxyribonucleic Acid Methylase Mutants of Escherichia coli K-12
- Nucleotide sequence of the dcm locus of Escherichia coli K12.
- Physical location of the ilvO determinant in Escherichia coli K-12 deoxyribonucleic acid.