Absence of 3′-Terminal Residues from Transfer Ribonucleic Acid of Dormant Spores of Bacillus megaterium

AUTOR(ES)

Setlow, Peter

RESUMO

Essentially all (>97%) of the transfer ribonucleic acid (tRNA) in log-phase and sporulating cells of Bacillus megaterium contains a complete 3′-cytidyl-cytidyl-adenosine terminus. However, about one-third of the tRNA in the dormant spore lacks the 3′-terminal adenosine 5′-monophosphate (AMP) residue, and some of the adjacent cytosine monophosphate residues are also missing. Examination of specific tRNAs indicated that those specific for isoleucine, leucine, and methionine are missing 30 to 40% of their terminal residue, whereas tRNAs specific for tyrosine lack 88% of the 3′-terminal AMP. Defective spore tRNA is not degraded during germination, but the missing residues are added back in the first minutes of the process. The enzyme catalyzing the addition reaction, tRNA nucleotidyltransferase, is present in the dormant spore at a level similar to that found in the vegetative cell.

Documentos Relacionados

• Percent Charging of Transfer Ribonucleic Acid and Levels of ppGpp and pppGpp in Dormant and Germinated Spores of Bacillus megaterium
• Messenger Ribonucleic Acid of Dormant Spores of Bacillus subtilis
• Isolation and characterization of two distinct fractions from the inner membrane of dormant Bacillus megaterium spores.
• Analysis of Isoaccepting Transfer Ribonucleic Acid Species of Bacillus subtilis: Chromatographic Differences Between Transfer Ribonucleic Acids from Spores and Cells in Exponential Growth
• INDUCED RELEASE OF DIPICOLINIC ACID FROM SPORES OF BACILLUS MEGATERIUM1