Absence of 5' terminal capping in encephalomyocarditis virus RNA.
AUTOR(ES)
Frisby, D
RESUMO
The nature of the 5' terminus of encephalomyocarditis (EMC) virion RNA has been investigated. We have failed to detect any capped products or nucleoside polyphosphates arising upon complete digestion of the RNA with T1, T2, and pancreatic ribonucleases, and it would therefore appear that the 5' terminus of EMC virus RNA is not phosphorylated and not capped with m7G. EMC virions do contain, however, large amounts of all four 5'-monosubstituted nucleoside triphosphates (4.2M pppG; 16.4M pppA; 3.OM pppU and 2.5M pppC), of which at least a proportion (about 15-20%) appear to remain bound to fully denatured RNA in the presence of divalent cations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=343127Documentos Relacionados
- Mapping 5' termini of JC virus late RNA.
- Defined mutations in the 5' nontranslated sequence of Sindbis virus RNA.
- Identification of the 5' end of Rous sarcoma virus RNA.
- The 5' terminal capping of heterogeneous nuclear RNA at different embryonic stages of the sea urchin.
- Size and location of poly (A) in encephalomyocarditis virus RNA.