Absence of Adenine-Rich Ribonucleic Acid from Purified Infectious Reovirus 3

AUTOR(ES)

Krug, Robert M.

RESUMO

The basic properties of released and cell-associated reovirus are the same. Both contained as their total nucleic acid complement only double-stranded ribonucleic acid (RNA) with an adenine content of 28%. Preparations of purified cell-associated virus, but not released virus, contained adenine-rich RNA which could be separated from the virus with little or no loss of infectivity. These adenine-rich ribonucleic acids were present in the virus preparations either as free RNA or associated with some structures of molecular weight less than 25 × 106 daltons. In contrast to our previous report, double-stranded reovirus RNA possessed little or no template activity for the Escherichia coli deoxyribonucleic acid and RNA polymerases.

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