Ação citotoxica e transformante do antineoplasico tamoxifeno sobre celulas da linhagem vero

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

Tamoxifen is a non-steroidal antiestrogen agent used as an adjuvant in the treatment of breast cancer and also in the preventive treatment of women with high potential risk to develop this disease. This medicament, despite its proved breast antineoplasic action, is associated to the increase of endometrial cancer, among others side effects. Therefore, there is the need of a wide investigation on the actions of the tamoxifen in the properties of different cellular types for a better comprehension of those actions. Vero cells line, which is non-tumoral, was used due to their growth and behavior pattern, being recommended for studies of cytotoxicity and cellular transformation. Hence, this work aimed to analyze the in vitro actions of the tamoxifen on Vero cells in culture, focusing cellular transformation. Vero cells were exposed at several tamoxifen concentrations, which has had cytotoxic and transformer actions, in a dose dependent manner. In immediate response to the treatment with 5 M tamoxifen / 24 hours Vero cells presented an increase in cellular proliferation whereas was observed a cytotoxic effect in more elevated concentrations. Treatment with 30 M tamoxifen/ 24 hours demonstrated to be the lower transformer dose and the cell line originated through this treatment, named Vero T30, was established for subsequent studies. This latter tamoxifen concentration can cause death by apoptosis in Vero cells but the remaining cells presented, differently from control, multi-layered growth; which is a behavior related to in vitro cellular transformation. The pattern of fibronectin distribution on normal and transformed cells was similar but the differences in the apoptosis rates, associated to the lost of contact inhibition can really indicate that there are two different cellular populations. Therefore, tamoxifen can cause in vitro morphological transformation, a phenomenon that can be related to in vivo neoplasic development by a process related to its toxicity.

ASSUNTO(S)

apoptose fibronectinas celula carcinogenese

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