Ação do perfluoroctano em cultura de celulas vero

AUTOR(ES)

Paulo Estacia

DATA DE PUBLICAÇÃO

1999

RESUMO

Culture of Vero cells was used as a model to evaluate the action of one of the .liquid perfluorocarbons (PFCL) most commonly used in ophthalmology in vitreo retinal surgeries, The Perfluoroctane (PFOC) has among other characteristics a high specific weight, which consequently facilitates the replacement of the retina. Analyses were made of the damage incurred to monolayers of Vero cells that were submitted to the action of PFOC, or the effect on the same confluent cells weighted down by a corresponding layer of glass coverslips that were equivalent to the PFOC weight and area. The cellular alterations were compared after 6,12 and 24 hours of contact with the PFOC or with the coverslips weight on the cellular monolayer The cells were evaluated with three methods: cresyl violet (CV) staining for morphological alterations; cytochemically, through staining with toluidine blue (TB) pH 4.0; and immunocytochemically, with the use of anti-actin and anti-vimentin antibodies. The results show damage to the cells, under the effect of weight, with cytoplamic retraction and formation of the cellular prolongations that bind the cells together. Also, a reduction in number of cells in the monolayer was observed but cells were found under the weight up to 24 hs of incubation. Cellular damage was greater in contact with PFOC, as the cells presented a more marked degeneration and the reduction of their number was more accentuated, with the alterations in alI cells in contact with PFOC after 24 hs. These results were more evident as the time of cell contact with PFOC or the coverslips weight increased. In the areas of the cellular monolayer that were not submitted to these factors no modification was observed. _llular alterations resulting ITom the physical effect caused by the high specific gravity of the PFOC were expected and were also found in cells under the effect of a proportional inert weight. The greater damage and the precocious alterations in the cells under the effect of the PFOC suggest a toxic interaction with the cells and not a reaction exc1usively due to weight. Consjdrring the damage that we found in cells submitted to the physical non-toxic effect of he weight of coverslips weight, the use of substances with a high specific weight to stabilize the retina over long periods of time seems to be limited. Moreover, the cellular damage verified in this study, which increased with time does not suggest the use of the PFOC as a vitreous substitute over long periods of time and shows the importance of its removal after the surgical procedure

ASSUNTO(S)

oftamologia

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