Acetylcholine receptor-inducing factor from chicken brain increases the level of mRNA encoding the receptor alpha subunit.

AUTOR(ES)

Harris, D A

RESUMO

A 42-kDa glycoprotein isolated from chicken brain, referred to as acetylcholine receptor-inducing activity (ARIA), that stimulates the rate of incorporation of acetylcholine receptors into the surface of chicken myotubes may play a role in the nerve-induced accumulation of receptors at developing neuromuscular synapses. Using nuclease-protection assays, we have found that ARIA causes a 2- to 16-fold increase in the level of mRNA encoding the alpha subunit of the receptor, with little or no change in the levels of gamma- and delta-subunit messengers. ARIA also increases the amount of a putative nuclear precursor of alpha-subunit mRNA, consistent with an activation of gene transcription. These results suggest that the concentration of alpha subunit may limit the rate of biosynthesis of the acetylcholine receptors in chicken myotubes. They also indicate that neuronal factors can regulate the expression of receptor subunit genes in a selective manner. Tetrodotoxin, 8-bromo-cAMP, and forskolin also increase the amount of alpha-subunit mRNA, with little change in the amount of gamma- and delta-subunit mRNAs. Unlike, ARIA, however, these agents have little effect on the concentration of the alpha-subunit nuclear precursor.

Documentos Relacionados

• A prion-like protein from chicken brain copurifies with an acetylcholine receptor-inducing activity.
• Acetylcholine receptor-inducing activity stimulates expression of the epsilon-subunit gene of the muscle acetylcholine receptor.
• A role for the acetylcholine receptor-inducing protein ARIA in oligodendrocyte development.
• Activity regulates the levels of acetylcholine receptor alpha-subunit mRNA in cultured chicken myotubes.
• Nucleotide sequence of the mouse muscle nicotinic acetylcholine receptor alpha subunit.