Acquired thermotolerance and heat shock proteins in thermophiles from the three phylogenetic domains.
AUTOR(ES)
Trent, J D
RESUMO
Thermophilic organisms from each of the three phylogenetic domains (Bacteria, Archaea, and Eucarya) acquired thermotolerance after heat shock. Bacillus caldolyticus grown at 60 degrees C and heat shocked at 69 degrees C for 10 min showed thermotolerance at 74 degrees C, Sulfolobus shibatae grown at 70 degrees C and heat shocked at 88 degrees C for 60 min showed thermotolerance at 95 degrees C, and Thermomyces lanuginosus grown at 50 degrees C and heat shocked at 55 degrees C for 60 min showed thermotolerance at 58 degrees C. Determinations of protein synthesis during heat shock revealed differences in the dominant heat shock proteins for each species. For B. caldolyticus, a 70-kDa protein dominated while for S. shibatae, a 55-kDa protein dominated and for T. lanuginosus, 31- to 33-kDa proteins dominated. Reagents that disrupted normal protein synthesis during heat shock prevented the enhanced thermotolerance.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=196839Documentos Relacionados
- Uncoupling thermotolerance from the induction of heat shock proteins.
- Acquired thermotolerance and heat shock in the extremely thermophilic archaebacterium Sulfolobus sp. strain B12.
- Thermotolerance of Isolated Mitochondria Associated with Heat Shock Proteins 1
- Heat shock protein synthesis and thermotolerance in Cataglyphis, an ant from the Sahara desert.
- Phylogenetic relationships of Bacillus thuringiensis delta-endotoxin family proteins and their functional domains.