Actinomycin D induced DNase I hypersensitivity and asymmetric structure transmission in a DNA hexadecamer.
AUTOR(ES)
Bishop, K D
RESUMO
DNase I cleavage rates and nmr chemical shifts are shown to change for DNA sequences distal to an intercalated actinomycin D molecule in a duplex hexadecamer upon drug binding. Both sets of observations suggest that the source of these changes is a DNA-mediated structural response. The nmr results imply the response is transmitted preferentially in a 5'-to-3' direction from the drug binding site. An inequivalent response of the two strands to a ligand-induced conformational change immediately suggests a mechanism for distinguishing the sense and antisense strands of DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=333724Documentos Relacionados
- Actinomycin D induced DNase I cleavage enhancement caused by sequence specific propagation of an altered DNA structure.
- Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I.
- Actinomycin D facilitates transition of AT domains in molecules of sequence (AT)nAGCT(AT)n to a DNAse I detectable alternating structure.
- Polymorphism in N-2-acetylaminofluorene induced DNA structure as revealed by DNase I footprinting.
- Chromatin structure and DNase I hypersensitivity in the transcriptionally active and inactive porcine tumor necrosis factor gene locus.