Action of ethyl and methyl methane sulfonates on DNA injection and genetic recombination in T7 bacteriophage.
AUTOR(ES)
Karska-Wysocki, B
RESUMO
After treatment with methyl or ethyl methane sulfonate, T7 amber mutants display a reduced capacity for recombination. Moreover, alkylation reduces recombination frequency involving markers on the right-hand side of the genetic map more than it reduces recombination frequency involving markers on the left-hand side. We interpret this to mean that alkylation can stop DNA injection at any point along the DNA molecule, and that T7 phage injects its DNA in a unique fashion starting from the end carrying the genes for early proteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=354868Documentos Relacionados
- DNA injection and genetic recombination of alkylated bacteriophage T7 in the presence of nalidixic acid.
- Genetic recombination of bacteriophage T7 DNA in vitro.
- Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA.
- Order of Injection of T7 Bacteriophage DNA
- High-molecular-weight DNA and the sedimentation coefficient: a new perspective based on DNA from T7 bacteriophage and two novel forms of T4 bacteriophage.
