Activation of Avena Coleoptile Cell Wall Glycosidases by Hydrogen Ions and Auxin 1
AUTOR(ES)
Johnson, Kenneth D.
RESUMO
Several cell wall-bound glycosidases present in Avena sativa coleoptiles were assayed by following the hydrolysis of p-nitrophenyl-glycosides. Particular emphasis was placed on characterizing some parameters affecting the activity of β-galactosidase. The pH optimum of this enzyme is 4.5 to 5.5; it is sensitive to copper ions and p-chloromercuribenzoate treatment and apparently has an exceptionally low turnover rate. Indoleacetic acid treatment enhanced in vivo β-galactosidase activity of coleoptile segments by 36% over control after 60 minutes. This enhancement was prevented by abscisic acid and cycloheximide. High buffer strengths and low pH reduced the indoleacetic acid-enhanced increase in enzyme activity. These data lend support to the following proposed model of indoleacetic acid action. Indoleacetic acid enhances the release of hydrogen ions into the cell wall which promote the activities of cell wall glycosidases, some of which may participate in the cell extension process.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=541368Documentos Relacionados
- Auxin-induced Changes in Avena Coleoptile Cell Wall Composition
- Rapid Inhibition of Auxin-induced Elongation of Avena Coleoptile Segments by Cordycepin 1
- Effect of Water Stress on Cell Wall Metabolism of Avena Coleoptile Tissue 1
- Auxin-Induced Water Uptake by Avena Coleoptile Sections. 12
- Turgor-dependent Changes in Avena Coleoptile Cell Wall Composition
