Activation of the alternative pathway of complement by monosodium urate monohydrate crystals and other inflammatory particles.
AUTOR(ES)
Doherty, M
RESUMO
Activation of serum C3 by monosodium urate monohydrate (MSU) crystals and other particles was determined by immunofixation following electrophoretic separation of C3 and its activation products. Densitometry allowed quantitation of results. MSU, hydroxyapatite, brushite, and calcium pyrophosphate dihydrate crystals split C3 under conditions which demonstrate activation via the alternative pathway (AP). Quantitatively similar results were obtained in immunoglobulin deficient serum. Activation was crystal specific and was reduced by heating, grinding, sonication, and aging of crystals. Other inflammatory particles (e.g., blackthorn) activated C3 via the AP: noninflammatory particles (e.g., diamond) caused insignificant activation. It is suggested that particle-induced activation of the alternative pathway of complement may be important in the initiation of crystal-induced synovitis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1001133Documentos Relacionados
- Immunoglobulin G independent activation of the classical complement pathway by monosodium urate crystals.
- Nucleation of monosodium urate crystals.
- Growth of monosodium urate monohydrate crystals: effect of cartilage and synovial fluid components on in vitro growth rates.
- Release of platelet constituents by monosodium urate crystals.
- C5a activation by monosodium urate crystals: effect of adsorption of serum and polymorphonuclear leucocyte homogenate
