Active repression of transcription by the engrailed homeodomain protein.
AUTOR(ES)
Jaynes, J B
RESUMO
The Drosophila engrailed gene product (En) is a homeodomain-containing protein that contributes to segmental patterning. In transfection assays it acts as a transcriptional repressor. We show that En is an active repressor, blocking activation by mammalian and yeast activators that bind to sites some distance away from those bound by En. Active repression is distinct from the effects of passive homeodomain-containing proteins, which repress when competing with activators for binding sites and activate when competing with En. Active repression activity maps outside the En homeodomain, and this activity can be transferred to a heterologous DNA binding domain.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=452804Documentos Relacionados
- Engrailed, a homeodomain protein, can repress in vitro transcription by competition with the TATA box-binding protein transcription factor IID.
- Mechanism of repression of RNA polymerase I transcription by the retinoblastoma protein.
- Functional domains of the Drosophila Engrailed protein.
- Involvement of Negative Cofactor NC2 in Active Repression by Zinc Finger-Homeodomain Transcription Factor AREB6
- Repression of bovine papillomavirus type 1 transcription by the E1 replication protein.