Active Transport of Calcium in Inverted Membrane Vesicles of Escherichia coli
AUTOR(ES)
Rosen, Barry P.
RESUMO
Accumulation of 45Ca++ was found to occur in membrane vesicles of E. coli prepared by lysis with a French pressure cell. The uptake occurs by active transport, requiring an energy source. Substrates of the electron transport chain, including D-lactate, reduced phenazine methosulfate, and NADH, stimulated accumulation, but this effect was blocked by the addition of cyanide. ATP could also stimulate accumulation, and this effect was blocked by dicyclohexylcarbodiimide. Uncouplers of oxidative phosphorylation inhibited the accumulation driven by either type of energy source.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=434036Documentos Relacionados
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