Active Transport of d-Alanine and Related Amino Acids by Whole Cells of Bacillus subtilis
AUTOR(ES)
Clark, Virginia L.
RESUMO
Whole cells of Bacillus subtilis transported d-alanine and l-alanine by two different systems. The high-affinity system (Km of 1 μM and Vmax of 0.6 to 0.8 nmol/min per mg of protein) was specific for the two stereoisomers of alanine. The low-affinity system (Km of 10 μM for l-alanine and 20 μM for d-alanine and glycine) had a Vmax of 5 to 12 nmol/min per mg of protein. This system transported glycine, d-cycloserine, and d-serine, in addition to d- and l-alanine. Azide inhibited the uptake of these amino acids and caused the efflux of d-alanine from preloaded cells. These data suggest that transport of these amino acids is energized by the electron transport chain.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=245886Documentos Relacionados
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