Active Transport of Exogenous S-Adenosylmethionine and Related Compounds into Cells and Vacuoles of Saccharomyces cerevisiae

AUTOR(ES)

Nakamura, K. D.

RESUMO

Saccharomyces cerevisiae 4094-B (α, ade-2, ura-1) in potassium phosphate buffer with glucose under aerobic conditions took up (−)S-adenosyl-l-methionine from the medium in sufficient quantity to permit the demonstration of its accumulation in the vacuole by ultraviolet micrography. The same result was obtained with (±)S-adenosyl-l-methionine, (±)S-adenosyl-d-methionine, and (−)S-adenosyl-l-ethionine. The rate of uptake was slow with (−)S-adenosyl-S(n-propyl)-l-homocysteine and S-adenosyl-d-homocysteine. S-Adenosyl-l-homocysteine was assimilated rapidly, but intracellular degradation precluded accumulation and ultraviolet micrographic studies. The uptake of 5′-methyl-, 5′-ethyl-, 5′-n-propylthioadenosine, and 5′-dimethylsulfonium adenosine was minimal.

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