Adeno-associated virus vectors preferentially transduce cells in S phase.

AUTOR(ES)

Russell, D W

RESUMO

Vectors based on adeno-associated virus can stably transfer genes by chromosomal integration in recipient cells. In this study we have infected stationary and dividing primary human fibroblast cultures with adeno-associated virus vectors encoding alkaline phosphatase and neomycin phosphotransferase. We find that the transduction frequency of S phase cells is about 200 times that of non-S phase cells. However, neither S phase nor mitosis is essential for transduction. Single-stranded vector genomes survive in stationary cultures and can be recruited for transduction by stimulating these cultures to divide. Stable transductants contain randomly integrated vector sequences. These findings have important implications for the use of adeno-associated virus vectors in gene therapy.

Documentos Relacionados

• Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells.
• Infectious Entry Pathway of Adeno-Associated Virus and Adeno-Associated Virus Vectors
• How adeno-associated virus Rep78 protein arrests cells completely in S phase
• Adeno-associated virus (AAV) vectors: will they work?
• Efficient gene transfer into nondividing cells by adeno-associated virus-based vectors.