Adenovirus 12S E1A gene represses differentiation of F9 teratocarcinoma cells.
AUTOR(ES)
Weigel, R J
RESUMO
The F9 teratocarcinoma cell line differentiates in vitro after treatment with retinoic acid and cAMP and has been a widely used model system for the study of the molecular events that are responsible for cellular commitment and differentiation during early development. Previous experiments have suggested intriguing parallels between the control of gene expression during F9 cell differentiation and the regulation of gene expression by adenovirus E1A. Transfection of a 12S E1A-expressing plasmid into terminally differentiated, nonproliferating F9 cells generates, at high frequency, colonies of dividing cells, each of which expresses E1A. Cell lines established from these colonies proliferate in the presence of retinoic acid and have lost the fully differentiated phenotype as characterized by the absence of expression of a series of differentiation-specific genes. We conclude that expression of the viral 12S E1A gene product interferes with retinoic acid-induced F9 cell differentiation. Moreover, the results suggest that the differentiation process, as defined by markers of terminal differentiation, may not be a permanent event but can be reversed by E1A expression.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=55277Documentos Relacionados
- The adenovirus Ela gene induces differentiation of F9 teratocarcinoma cells.
- Transcriptional regulation of the c-jun gene by retinoic acid and E1A during differentiation of F9 cells.
- Phosphorylation of the adenovirus E1A-associated 300 kDa protein in response to retinoic acid and E1A during the differentiation of F9 cells.
- Antisense Myc sequences induce differentiation of F9 cells.
- Selective induction of human heat shock gene transcription by the adenovirus E1A gene products, including the 12S E1A product.