Adenovirus Messenger RNA in Mammalian Cells: Failure of Polyribosome Association in the Absence of Nuclear Cleavage

AUTOR(ES)

McGuire, P. M.

RESUMO

The nuclear synthesis of adenovirus-specific RNA late in the infectious cycle in the presence of toyocamycin (an adenosine analogue) has been investigated. There is reduced synthesis of viral RNA with an accumulation of virus-specific RNA in the molecular weight range of at least 4 to 8 × 106. No new viral RNA associates with cytoplasmic polyribosomes. In addition, hybridization competition experiments indicate a 70% competition between these large nuclear transcripts and polyribosome-associated viral RNA that was synthesized in the absence of inhibitor. These data are consistent with the following interpretations: complete nuclear processing of viral RNA is necessary for polyribosome association, and precursor viral message(s) contain sequences that are lost normally during post-transcriptional processing.

Documentos Relacionados

• Messenger and Heterogeneous Nuclear RNA in HeLa Cells: Differential Inhibition by Cordycepin*
• Messenger RNA deadenylylation precedes decapping in mammalian cells
• Rapidly labeled, polyribosome-associated RNA having the properties of histone messenger.
• Elongation of the Polyadenylate Segment of Messenger RNA in the Cytoplasm of Mammalian Cells
• Multiple ribosomal RNA cleavage pathways in mammalian cells