Adenovirus type 2 late mRNA's: structural evidence for 3'-coterminal species.
AUTOR(ES)
Ziff, E
RESUMO
Adenovirus type 2-infected HeLa cells were labeled with 32PO4 during the period 14 to 17 h postinfection. Viral mRNA's with polyadenylic acid were isolated by polyuridylic acid Sepharose chromatography and fractionated according to size by electrophoresis through an acrylamide-agarose slab gel. Messenger bands were eluted and partially degraded with alkali. RNA fragments from each band that contain polyadenylic acid were isolated by polyuridylic acid Sepharose chromatography and fingerprinted two-dimensionally after T1 RNase digestion. Three bands, with mobilities of approximately 26S, 21S, and 18S, shared two large characteristic T1 oligonucleotides in common in the fingerprints of their 3'-terminal sequences. These oligonucleotides were mapped with a Hpa II restriction fragment of adenovirus type 2 DNA with coordinates 49-50.2. We conclude that the three mRNA's are coterminal in sequence at their 3' ends and overlap at internal positions. Implications for the protein-coding potential of these mRNA's and the mechanisms of adenovirus tyep 2 late RNA processing are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=525984Documentos Relacionados
- The adenovirus L4 100-kilodalton protein is necessary for efficient translation of viral late mRNA species.
- Multiple mRNA species for adenovirus type 2 polypeptides III and pVII.
- Adenovirus type 2 fiber mRNA synthesis: no evidence for a cytoplasmic processing pathway.
- Polyoma virus has three late mRNA's: one for each virion protein.
- Two "early" mRNA species in adenovirus type 2-transformed rat cells.
