Adherence of staphylococcus aureus to influenza A virus-infected Madin-Darby canine kidney cell cultures.
AUTOR(ES)
Davison, V E
RESUMO
Madin-Darby canine kidney cells infected with influenza A virus (strains PR8, FM1, Jap 305, and Tex 1) were tested with nine strains of Staphylococcus aureus and group B Streptococcus type Ic to determine whether mammalian cells become susceptible to bacterial adherence as a result of virus infection. Bacterial adherence to virus-infected cells varied depending on the virus strain and on the strain of bacteria tested. A quantitative radioassay was developed to study the parameters of adherence. Attachment of 3H-labeled S. aureus grown in chemically defined or biologically complex medium to FM1 virus-infected cells was significantly increased (P less than 0.0005) compared with attachment to control cells. Adherence coincided with the appearance of hemadsorption, which is a marker of the presence of virus-induced glycoproteins on the cell surface. Adherence was temperature dependent, increased with a decrease in hydrogen ion concentration, and was not affected by the presence of K+, Mg2+, or Ca2+. Adherence was blocked when 3H-labeled S. aureus was pretreated with trypsin but not when cells were pretreated with neuraminidase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=350596Documentos Relacionados
- Factors Influencing Adherence of Staphylococcus aureus to Influenza A Virus-Infected Cell Cultures
- Fibrinogen-mediated adherence of group A Streptococcus to influenza A virus-infected cell cultures.
- Actin Dependence of Polarized Receptor Recycling in Madin-Darby Canine Kidney Cell EndosomesV⃞
- TGN38 recycles basolaterally in polarized Madin-Darby canine kidney cells.
- Development of cell surface polarity in the epithelial Madin-Darby canine kidney (MDCK) cell line.