Adhesion of Escherichia coli strains isolated from diarrheic weaned rabbits to intestinal villi and HeLa cells.
AUTOR(ES)
Milon, A
RESUMO
Thirty-eight strains, representative of 575 Escherichia coli isolates from weaned diarrheic rabbits, were tested for their ability to adhere in vitro to rabbit intestinal villi and to HeLa 229 cells. The O103 rhamnose-negative, highly pathogenic strains, which are epidemiologically predominant in France, attached to intestinal villi prepared from 8-day-old as well as 6-week-old rabbits and gave a diffuse adhesion pattern with HeLa cells. These adhesion properties were associated with the presence of a protein with a molecular weight of 32,000 in surface extracts of the strains. The expression of the adhesion was dependent on culture medium and temperature, and the adhesion was D-mannose resistant. Antisera raised against the 32,000-molecular-weight protein inhibited adhesion. This adhesion was not expressed in two nonpathogenic O103 strains, indicating its implication in virulence. However, the same adhesin was expressed by two O128 non- or moderately pathogenic strains. Therefore, adhesion to enterocytes is not the only factor involved in the pathogenicity of O103 strains.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=258876Documentos Relacionados
- Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells.
- Pathogenic properties of Escherichia coli strains isolated from diarrheic commercial rabbits.
- Pathogenic Properties of Escherichia coli Strains Isolated from Diarrheic Commerical Rabbits
- pH changes during in vitro adherence of Escherichia coli to HeLa cells.
- Nascent ribosomes from HeLa cells.
