Agglutination of Bacterial Spheroplasts: Agglutination-Dependent Degradation of Escherichia coli Ribosomal Ribonucleic Acid
AUTOR(ES)
Maruyama, Hiromi B.
RESUMO
When Escherichia coli spheroplasts made by ethylenediaminetetraacetic acid and lysozyme were agglutinated by concanavalin A (con A), the degradation of ribosomal ribonucleic acid (rRNA) was found to occur proportionally to the degree of the agglutination, which was determined by microscopic examination or by a newly devised assay based on the slower settling of aggregates. Methyl-α-d-glucoside, low temperature or alkaline pH, all of which reverse the agglutination, also reduced the extent of rRNA degradation. This degradation was not due to the direct action of con A since a similar relationship was found in the case of spontaneous agglutination with concentrated spheroplasts in the absence of con A. The possible importance of a change in the cell membrane associated with the agglutination process is discussed in connection with the initiation of rRNA degradation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=246210Documentos Relacionados
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