Aggregative adherence of Klebsiella pneumoniae to human intestine-407 cells.
AUTOR(ES)
Favre-Bonte, S
RESUMO
Aggregative adhesion of Klebsiella pneumoniae LM3 to Intestine-407 (Int-407) cells was studied. Adhesive capacities were affected by the bacterial growth phase (with a maximum of adherence obtained during the exponential phase), temperature, multiplicity of infection, and length of incubation with Int-407 cells. Adhesion occurred through a cytochalasin D-sensitive process and was greatly reduced after treatment of Int-407 with cycloheximide, indicating that aggregative adhesion requires active participation of Int-407 cells. Transmission electron microscopy revealed that adherent bacteria were surrounded by a capsule-like material, apparently involved in both bacterium-Int-407 cell and bacterium-bacterium adherence. Examination with a scanning electron microscope showed interactions of intestinal cell microvilli with bacteria and formation in 3 h of a fibrous network within and around the bacterial clusters. We speculate that aggregative adhesion of K. pneumoniae mediated by a capsule-like extracellular material might explain the persistence of these strains inside the host gastrointestinal tract.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=173153Documentos Relacionados
- Aeromonas caviae exhibits aggregative adherence to HEp-2 cells.
- Aeromonas species exhibit aggregative adherence to HEp-2 cells.
- Adherence of Giardia lamblia Trophozoites to Int-407 Human Intestinal Cells
- Adherence of Giardia lamblia Trophozoites to Int-407 Human Intestinal Cells
- Shiga toxin-producing Escherichia coli isolates from cases of human disease show enhanced adherence to intestinal epithelial (Henle 407) cells.