Agrobacterium tumefaciens DNA and PS8 Bacteriophage DNA Not Detected in Crown Gall Tumors
AUTOR(ES)
Chilton, Mary-Dell
RESUMO
Renaturation kinetics of labeled Agrobacterium tumefaciens DNA are not influenced by addition of 104-fold excess of crown gall tumor DNA. Reconstruction experiments demonstrated that 0.01% added bacterial DNA produces a detectable increase in rate of renaturation of labeled DNA. Crown gall tumor DNA therefore cannot contain as much as 0.01% A. tumefaciens DNA (one entire bacterial genome per three diploid tumor cells).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433838Documentos Relacionados
- Attempts to Detect Deoxyribonucleic Acid from Agrobacterium tumefaciens and Bacteriophage PS8 in Crown Gall Tumors by Complementary Ribonucleic Acid/Deoxyribonucleic Acid-Filter Hybridization
- On the Question of Crown-Gall Tumor Initiation by DNA of Bacteriophage PS8
- Methylation of the T-DNA in Agrobacterium tumefaciens and in several crown gall tumors.
- Proteins encoded by Agrobacterium tumefaciens Ti plasmid DNA (T-DNA) in crown gall tumors
- Genetic analysis of nonpathogenic Agrobacterium tumefaciens mutants arising in crown gall tumors.
