all-trans-Retinal stimulates superoxide release and phospholipase C activity in neutrophils without significantly blocking protein kinase C.

AUTOR(ES)

Lochner, J E

RESUMO

all-trans-Retinal was previously shown to stimulate high levels of superoxide release by guinea pig neutrophils. When the cells, previously labeled with [3H]inositol, are treated with all-trans-retinal, they exhibit a decrease in the levels of [3H]inositol phospholipids and an increase in the accumulation of [3H]inositol phosphates. The maximal accumulation of inositol phosphates and the optimal rate of superoxide release occurred together at approximately 7 min after stimulation. The levels of [3H]inositol phosphates accumulated were comparable to those observed when the cells were stimulated with a chemotactic peptide. In direct measurements, using concentrations that stimulate intact cells maximally, all-trans-retinal was found not to inhibit protein kinase C from the cytosol of neutrophils significantly. This contrasts with the situation with this kinase obtained from other sources. These observations represent additional effects of vitamin A on cells.

Documentos Relacionados

• Involvement of All-trans-retinal in Acute Light-induced Retinopathy of Mice*S⃞
• All-trans-retinal shuts down rod cyclic nucleotide-gated ion channels: A novel role for photoreceptor retinoids in the response to bright light?
• Dopamine induces neurite retraction in retinal horizontal cells via diacylglycerol and protein kinase C.
• Phospholipase C-gamma 1 binding to intracellular receptors for activated protein kinase C.
• Transformation stimulates glucose transporter gene expression in the absence of protein kinase C.