Allele-specific methylation and expression of an imprinted U2af1-rs1 (SP2) gene.

AUTOR(ES)

Hatada, I

RESUMO

The mouse U2af1-rs1(SP2) gene, which was cloned by a two-dimensional genome scanning method, is expressed exclusively from the paternally inherited chromosome. This gene has significant similarity to U2AF and located in chromosome 11, of which maternal duplication/paternal deficiency results in a small body. In this report, we cloned genomic U2af1-rs1(SP2) and found its promoter was methylated in a maternal-allele-specific manner. This allelic methylation was not established in parental gametes, but established between 1.5 d.p.c. and 12.5 d.p.c. on the contrary, the allele-specific expression occurred in the two-cell stage when transcription initiates. Absence of the methylation of the upstream region in this stage indicates that methylation is not necessary for inactivation of the expression.

Documentos Relacionados

• Mouse U2af1-rs1 is a neomorphic imprinted gene.
• Allele-specific histone lysine methylation marks regulatory regions at imprinted mouse genes
• The ontogeny of allele-specific methylation associated with imprinted genes in the mouse.
• DNA Methylation Is Linked to Deacetylation of Histone H3, but Not H4, on the Imprinted Genes Snrpn and U2af1-rs1
• Simultaneous genotyping, gene-expression measurement, and detection of allele-specific expression with oligonucleotide arrays