Alteration of cellular gene expression in adenovirus transformed cells by post-transcriptional mechanisms.
AUTOR(ES)
Kao, H T
RESUMO
We have isolated cDNA clones complementary to human mRNAs that are expressed at elevated levels in 293 cells, adenovirus-transformed human embryonic kidney cells, as compared to a normal counterpart of this cell line. Approximately 200 clones out of 100,000 that were screened were positive; 40 of these were isolated, of which 31 were determined to be unique and were further characterized. Each clone detected a mRNA that was 5 to 50 times more abundant in 293 cells than in the non-transformed HEK cell line. For several of these transcripts, the elevated expression appeared to be a function of transformation since they were also high in other human tumor cell lines. Strikingly, we have found that post-transcriptional control is largely responsible for the regulation of the abundance of these mRNAs.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=311750Documentos Relacionados
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