Alterations in gene expression during heat shock of Achlya ambisexualis.
AUTOR(ES)
Gwynne, D I
RESUMO
When exponentially growing cultures of Achlya ambisexualis strain E87 were raised from their normal growth temperature of 30 degrees C to 35 degrees C, the rates of synthesis of a small number of proteins were dramatically increased. The most predominant proteins synthesized in response to heat shock had molecular weights of 70,000 and 78,000, and their increased synthesis was detected as early as 10 min after the shift to 35 degrees C. Changes in the populations of translatable messenger RNAs during heat shock showed that the levels of the mRNA's for all the major induced proteins correlated very closely with the alterations in the in vivo patterns, suggesting a transcriptional level of control of their synthesis. When after a period of heat shock (60 min) the cultures were shifted back to 30 degrees C, recovery of the preshock patterns of protein synthesis was attained after several hours. Different proteins show temporally distinct patterns of recovery. During recovery the levels of translatable mRNA's for the induced proteins also correlated closely with the patterns of in vivo protein synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=216532Documentos Relacionados
- Changes in chromatin and the phosphorylation of nuclear proteins during heat shock of Achlya ambisexualis.
- Restriction endonuclease mapping by crossed contact hybridization: the ribosomal RNA genes of Achlya ambisexualis.
- Steroid hormone regulation of the Achlya ambisexualis 85-kilodalton heat shock protein, a component of the Achlya steroid receptor complex.
- Cellulase Localization in Hyphae of Achlya ambisexualis
- Sequence requirement for expression of the Drosophila melanogaster heat shock protein hsp22 gene during heat shock and normal development.