Altered pattern of replication of human chromosomes in a human fibroblast-mouse cell hybrid.
AUTOR(ES)
Farber, R A
RESUMO
The pattern of terminal replication of the human chromosomes in a clone of hybrids between diploid human fibroblasts and mouse cells was analyzed by autoradiography. An average of 10 human chromosomes was present in the hybrid cells. Several of these chromosomes were found to terminate replication in a different order from the same chromosomes in the parental human fibroblasts. Chromosomes 4 and 5 completed replication later in the hybrid than in the fibroblasts (relative to the other human chromosomes). In contrast, chromosomes 7, 12, and 15 completed replication earlier in the hybrid than in the fibroblasts. These results suggest that the sequence of terminal chromosome replication in human fibroblasts is not irreversibly programmed into each chromosome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=411494Documentos Relacionados
- Expression of human neuronal antigens in a mouse neuroblastoma x human dorsal root ganglion cell hybrid.
- Pattern of Immunoglobulin Synthesis and Assembly in a Human-Mouse Somatic Cell Hybrid Clone
- Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes.
- Oncornavirus Expression in Human × Mouse Hybrid Cells Segregating Mouse Chromosomes
- More than one-third of the discernible mouse polypeptides are not expressed in a Chinese hamster-mouse embryo fibroblast hybrid that retains all mouse chromosomes.
