Altered shape and cell cycle characteristics of fibroblasts expressing the E2F1 transcription factor.
AUTOR(ES)
Logan, T J
RESUMO
To gain an understanding of the role the E2F1 transcription factor plays in cell physiology, the full length protein (E2F1) and an amino terminal deletion of 87 amino acids (E2F1d87) were constitutively expressed in NIH3T3 fibroblasts. Multiple cell lines were generated for each construct. These cells do not proliferate in media containing low serum and do not proliferate in soft agar, indicating that they are likely not transformed. However, both sets of cell lines show increased DNA synthesis and increased numbers of cells in S phase when cultured in media containing low serum, compared to the control cell lines. Cells expressing E2F1d87 (but not E2F1) have an extremely rounded morphology when cultured in 10% serum-containing media. These rounded cells lack detectable microfilaments, microtubules, and focal contacts. However, when these cells are cultured in low serum-containing media (0.5%), they attain the flattened morphology and cytoskeletal structure of normal NIH3T3 cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=301082Documentos Relacionados
- Physical and functional interactions between p53 and cell cycle co-operating transcription factors, E2F1 and DP1.
- Functional synergy between DP-1 and E2F-1 in the cell cycle-regulating transcription factor DRTF1/E2F.
- Interaction of Sp1 with the growth- and cell cycle-regulated transcription factor E2F.
- Expression of the E2F1 transcription factor overcomes type beta transforming growth factor-mediated growth suppression.
- Cell cycle-regulated association of E2F1 and Sp1 is related to their functional interaction.
