Alternative sites of transcription initiation upstream of the canonical cap site in human gamma-globin and beta-globin genes.
AUTOR(ES)
Grindlay, G J
RESUMO
Using S1 mapping and primer extension analysis, we have identified a number of human kappa-globin and beta-globin 5' RNA termini originating in the 200 bp upstream of the canonical mRNA cap sites. Upstream initiation sites have previously been reported for the human epsilon-globin gene (4,5) and the present work indicates that this is a general feature of the human beta-type globin genes. We have attempted to identify features common to such sites between the three genes. One site 170 bp upstream of the major beta-globin cap site and a site 1400 bp upstream of the major epsilon-globin cap site are located near putative PolIII promoter sequences and may therefore be transcribed by this enzyme. Alternative initiation sites located 200 bp and 50-100 bp upstream of the epsilon-globin and kappa-globin cap sites respectively are located within S1 hypersensitive regions of chromatin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=318622Documentos Relacionados
- Upstream G gamma-globin and downstream beta-globin sequences required for stage-specific expression in transgenic mice.
- Roles of fetal G gamma-globin promoter elements and the adult beta-globin 3' enhancer in the stage-specific expression of globin genes.
- Cloning and characterization of DNA sequences surrounding the human gamma-, delta-, and beta-globin genes.
- Genes for gamma-globin in human adult erythroid DNA.
- Developmental regulation of human gamma-globin genes in transgenic mice.
