Ameloblastos secretores : secreção, absorção e reutilização de amelogenina marcada com 3H-prolina, visualizadas atraves de radioautografia

AUTOR(ES)
DATA DE PUBLICAÇÃO

1987

RESUMO

Two groups of mice 12 hrs old were sacrificed from 1 to 144 hours after the injection of 3H-Thymidine or 3H-Proline. One group of 21 animaIs, each weighing on average 1,5 g.were injected with a single dose of 1 ~Ci/g body weight of 3H-Thymidine and the other group of 27 animaIs each weighing on average 1,4 g.received a single dose of 5 ~Ci/g body weight of 3H-proline. The unfixed and undecalcified right half mandibles, co taining one incisor tooth were embedded in Tissue-Teck II using gelatin capsules and were immediately frozen. Transverse sections,6 ~m in thickness, were cut on a cryotome at -27oC beginning at the incisal tip or at the odontogenic organ of the tooth. The fixed and undecalcified left half mandibles were embedded in Poly bed 812 and cut to obtain longitudinal sections of 1 ~m thickness. Radioautography was carried out using a coating technique with Ilford K5 - emulsion...Note: The complete abstract is available with the full electronic digital thesis or dissertations

ASSUNTO(S)

autoradiografia patologia bucal

Documentos Relacionados