An analysis of T-cell receptor variable region gene expression in major histocompatibility complex disparate mice.
AUTOR(ES)
Bill, J
RESUMO
To define the impact of major histocompatibility complex (MHC)-encoded glycoproteins on the selection of the T-cell receptor repertoire, we have determined the frequency with which T-cell receptor variable region (V alpha and V beta) genes are expressed in T cells from MHC disparate mice. Approximately 500 T-cell hybridomas were generated from each of three strains of MHC congenic mice [B10 (H-2b), B10.BR (H-2k), and B10.Q (H-2q)] by fusing mitogen-stimulated lymph node T cells with the thymoma BW5147. RNA was prepared from 1629 individual hybridomas and analyzed for the expression of 10 V alpha and 16 V beta gene families. These experiments reveal significant differences in the relative contributions of 1 V alpha gene family (V alpha 3) and several V beta gene segments (V beta 5.1, -5.2, -11, and -12) to the T-cell receptor repertoire of MHC disparate mice.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=282701Documentos Relacionados
- T-cell receptor variable region gene usage in T-cell populations
- T-cell receptor variable region gene usage in T-cell populations.
- Major histocompatibility complex determinants select T-cell receptor alpha chain variable region dominance in a peptide-specific response.
- T-cell specific rearrangement of T-cell receptor beta transgenes in mice.
- Thymic depletion and peripheral activation of class I major histocompatibility complex-restricted T cells by soluble peptide in T-cell receptor transgenic mice.
