An in vitro system from Plasmodium falciparum active in endogenous mRNA translation
AUTOR(ES)
Ferreras, Ana, Triana, Ledia, Correia, Heriberto, Sánchez, Erlinda, Herrera, Flor
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2000-04
RESUMO
An in vitro translation system has been prepared from Plasmodium falciparum by saponin lysis of infected-erythrocytes to free parasites which were homogeneized with glass beads, centrifuged to obtain a S-30 fraction followed by Sephadex G-25 gel filtration. This treatment produced a system with very low contamination of host proteins (<1%). The system, optimized for Mg2+ and K+, translates endogenous mRNA and is active for 80 min which suggests that their protein factors and mRNA are quite stable.
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