An induction gene trap screen in embryonic stem cells: Identification of genes that respond to retinoic acid in vitro.
AUTOR(ES)
Forrester, L M
RESUMO
We have developed a novel induction gene trap approach that preselects in vitro for integrations into genes that lie downstream of receptor/ligand-mediated signaling pathways. Using this approach, we have identified 20 gene trap integrations in embryonic stem cells, 9 of which were induced and 11 of which were repressed after exposure to exogenous retinoic acid (RA). All but one of these integrations showed unique spatially restricted or tissue-specific patterns of expression between 8.5 and 11.5 days of embryogenesis. Interestingly, expression was observed in tissues that are affected by alterations in RA levels during embryogenesis. Sequence analysis of fusion transcripts from six integrations revealed five novel gene sequences and the previously identified protooncogene c-fyn. To date, germ-line transmission and breeding has uncovered one homozygous embryonic lethal and three homozygous viable insertions. These studies demonstrate the potential of this induction gene trap approach for identifying and mutating genes downstream of signal transduction pathways.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=40001Documentos Relacionados
- Mouse embryonic stem cells: the establishment of the system to produce differentiated cell types in vitro
- Bovine cumulus-granulosa cells contain biologically active retinoid receptors that can respond to retinoic acid
- Identification of four murine cDNAs encoding putative protein kinases from primitive embryonic stem cells differentiated in vitro.
- Retinoic acid enhances growth of human early erythroid progenitor cells in vitro.
- Selective Disruption of Genes Transiently Induced in Differentiating Mouse Embryonic Stem Cells by Using Gene Trap Mutagenesis and Site-Specific Recombination