An Inhibitor of Histamine Release from Human Leukocytes

AUTOR(ES)

Kelly, Michael T.

RESUMO

Lysates of human leukocytes, prepared by ultrasonic disruption, contained heat-labile histamine-releasing factors. An inhibitor of histamine release was generated from such lysates during thermal inactivation of the histamine-releasing activity. Lysates inactivated at 38°C for 60 min produced up to 90% inhibition of histamine release from human leukocytes. Heated lysates produced significant inhibition (P < 0.001) of leukocyte histamine release induced by goat antihuman IgE (51±3% inhibition), compound 48/80 (69±7% inhibition), and fresh leukocyte lysate (63±6% inhibition).

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