An ordered collection of Bacillus subtilis DNA segments cloned in yeast artificial chromosomes.
AUTOR(ES)
Azevedo, V
RESUMO
A collection of 772 Bacillus subtilis DNA segments was obtained by cloning in yeast artificial chromosomes. The B. subtilis inserts of 288 clones were mapped by hybridization using as probes 65 cloned genes and 188 isolated insert ends. In this way, 59 inserts were ordered in four contigs that cover > 98% of the B. subtilis chromosome. This ordered collection is now available for further genetic and physical analysis of the B. subtilis genome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=46864Documentos Relacionados
- Mitotic recombination of yeast artificial chromosomes.
- Regional localization on the human X of DNA segments cloned from flow sorted chromosomes.
- DNA class organization on maize Adh1 yeast artificial chromosomes.
- A method for linking yeast artificial chromosomes.
- Construction of human chromosome 21-specific yeast artificial chromosomes.
