An upstream uncD sequence modulates translation of Escherichia coli uncC.
AUTOR(ES)
Dunn, S D
RESUMO
The effect of upstream uncD sequences on expression of the Escherichia coli uncC gene, encoding the epsilon subunit of F1-ATPase, was studied. uncC expression was reduced severalfold in plasmid constructs bearing, in addition to uncC, a region of uncD located between 85 and 119 bases upstream from the uncC initiation codon. This reduction was independent of in-frame translation of the uncD sequences. An mRNA stem-loop structure in which sequences located within the inhibitory region of uncD base pair with the uncDC intercistronic region is suggested to function in modulating uncC expression.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=208927Documentos Relacionados
- Translation through an uncDC mRNA secondary structure governs the level of uncC expression in Escherichia coli.
- Altered translation of the uncC gene coding for the epsilon subunit of the F1F0-ATPase of Escherichia coli.
- Expression of Escherichia coli infC: identification of a promoter in an upstream thrS coding sequence.
- Construction and characterization of an Escherichia coli strain with a uncI mutation.
- Signal sequence mutations that alter coupling of secretion and translation of an Escherichia coli outer membrane protein.