Análise da acetona em ar exalado: metodologia para estudo em pacientes hospitalizados / Breath Acetone Analysis: Methodology for studying hospitalized pacients

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

The dissertation fits in the research field of noninvasive clinical analysis, more specifically, the prognosis of the severity of heart failure, HF, based on a biomarker found in the air exhaled by patients, in a study at InCor-HCFMUSP. The most relevant biomarker found by GC-MS was acetone (propanone). For the breath collection a device was developed which comprises a scrubber flask (impinger) with diffuser, containing 5 mL of water, immersed in an ice bath and attached to a plastic bag for delimitation of the sample volume (7,6 L). For the determination of acetone a spectrometric method (474 nm) was chosen based on a reaction of acetone with salicylaldehyde in alkaline medium, with detection limits of 0,3 mg/L in liquid phase and 0,3 µg/L in breath. Preliminary studies made with samples collected from HF patients by the co-workers from InCor indicate the potential of the identified biomarker and the suitability of the equipment and methods developed in this dissertation for its analysis.

ASSUNTO(S)

acetona acetone amostragem de gases análise de ar exalado analytical chemistry biomarcadores breath analysis espectrofotometria gas sampling método não-invasivo non- invasive analysis pacientes internados (análise química) química anatítica spectrophotometry

ACESSO AO ARTIGO

Documentos Relacionados