Análise do papel do gene cspC de Caulobacter crescentus e de sua regulação. / Study of the role cspC gene from Caulobacter crescentus and its regulation.
AUTOR(ES)
Heloise Balhesteros
DATA DE PUBLICAÇÃO
2009
RESUMO
The cold shock response in bacteria involves the expression of cold shock proteins (CSPs), which destabilize secondary structures on mRNAs, allowing their translation. Caulobacter crescentus possesses four genes encoding CSPs: cspA and cspB are induced upon cold shock, while cspC and cspD are induced at stationary phase. In this work, a new sequence for the coding region of the cspC gene was determined, revealing that CspC contains two cold shock domains, like CspD. A null cspC mutant was sensitive to low temperature, presented reduced viability at stationary phase, and altered morphology. The regulatory region of cspC was mapped by transcriptional fusions, identifying a region responsible for activation of cspC expression, suggesting a transcriptional regulation. Some nutritional conditions triggering cspC induction were determined, indicating that its expression is influenced by glucose starvation, but not by nitrogen starvation. This expression profile was not dependent on the activation region, which, in turn, was required for maximum levels of expression.
ASSUNTO(S)
gene regulation biologia molecular microbiologia proteínas de choque frio bacterial stress response caulobacter crescentus microbiology caulobacter crescentus cold shock proteins molecular biology resposta a estresse em bactérias regulação gênica
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