AnÃlise genÃmica e pÃs-genÃmica de proteÃnas de Leishmania chagasi

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

Leishmania are intracellular parasites that cause a large spectrum of diseases, ranging from single cutaneous lesions to fatal visceral infections. In spite of the progress towards the understanding of the epidemiology, immunology and biochemistry of the leishmaniases, an increase in new cases is observed worldwide. Recently a large set of Leishmania chagasi cDNA sequences were made available by the Northeast Brazilian Genome Project. In this work we briefly analyze the distribution of the first 10,000 sequences in functional categories, the GC content of coding and non-coding sequences and identified a set of genes restricted to trypanosomatids. We also analyzed two conserved genes, hsp70 and hsp83, whose full length cDNA sequences were available. Recombinant polypeptides containing progressively larger HSP70 sequences were used in an immunoadsorption experiment to identify antigenic regions over the full range of the protein. Moreover, the deduced aminoacid sequences for HSP70 and HSP83 were compared with a large set of similar sequences among trypanosomatids, as well as other taxa, and divergent regions were clearly associated with epitopes or antigenic regions, defined in this work or elsewhere. Based of a comparative study of 5â-UTR and 3â-UTR, and of the corresponding genomic sequences of hsp83 and of other genes available for L. chagasi, as well as for other Leishmania species, we identified the precise trans-splicing and poly-adenilation sites for hsp83. This work offers, therefore, an important contribution towards the understanding of the antigenicity of HSP in parasitic and microbial infections and to the definition of mRNA processing sites in Leishmania

ASSUNTO(S)

epitopo de linfÃcito b hsp83 leishmania chagasi leishmaniose visceral hsp70 sorodiagnÃstico microbiologia trans-encadeamento poli-adenilaÃÃo

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