Analysis of Immunoglobulin A Antibodies to Helicobacter pylori in Serum and Gastric Juice in Relation to Mucosal Inflammation

AUTOR(ES)

Hayashi, Shunji

FONTE

American Society for Microbiology

RESUMO

Helicobacter pylori is a major etiologic agent in gastroduodenal disorders. In this study, immunoglobulin A (IgA) antibodies to H. pylori antigens were evaluated in serum and gastric juice specimens obtained from patients with gastritis or peptic ulcers by utilizing antibody capture enzyme-linked immunosorbent assays (ACELISAs). Urease α subunit (UA), urease β subunit (UB), the 66-kDa heat shock protein (HSP), and the 25-kDa protein (25K) were used as antigens for the ACELISAs. The antibody titers of the ACELISAs reflect the ratio of H. pylori-specific IgA to total IgA. The ratio is stable, although the antibody concentration fluctuates in gastric juice. By using ACELISAs it was possible to evaluate quantitatively not only serum IgA antibodies but also gastric juice secretory IgA (S-IgA) antibodies. In both serum IgA and gastric juice S-IgA ACELISAs, the titers of antibody to HSP and 25K were remarkably correlated with the histologic grade of gastritis, whereas those to UA and UB were not strongly correlated with histologic grade. Thus, it is useful for estimating the histologic grade of gastritis to quantify serum IgA and gastric juice S-IgA antibodies to HSP and 25K.

Documentos Relacionados

• Diagnosis of Helicobacter pylori infection by specific gastric mucosal IgA and IgG pylori antibodies.
• Immunoglobulin A antibodies to Helicobacter pylori.
• Rapid genetic analysis of Helicobacter pylori gastric mucosal colonization in suckling mice
• Helicobacter pylori does not release cysteamine into gastric juice.
• Use of a Gastric Juice-Based PCR Assay To Detect Helicobacter pylori Infection in Culture-Negative Patients