Analysis of Meiotic Recombination Pathways in the Yeast Saccharomyces Cerevisiae
AUTOR(ES)
Mao-Draayer, Y.
RESUMO
In the yeast, Saccharomyces cerevisiae, several genes appear to act early in meiotic recombination. HOP1 and RED1 have been classified as such early genes. The data in this paper demonstrate that neither a red1 nor a hop1 mutation can rescue the inviable spores produced by a rad52 spo13 strain; this phenotype helps to distinguish these two genes from other early meiotic recombination genes such as SPO11, REC104, or MEI4. In contrast, either a red1 or a hop1 mutation can rescue a rad50S spo13 strain; this phenotype is similar to that conferred by mutations in the other early recombination genes (e.g., REC104). These two different results can be explained because the data presented here indicate that a rad50S mutation does not diminish meiotic intrachromosomal recombination, similar to the mutant phenotypes conferred by red1 or hop1. Of course, RED1 and HOP1 do act in the normal meiotic interchromosomal recombination pathway; they reduce interchromosomal recombination to ~10% of normal levels. We demonstrate that a mutation in a gene (REC104) required for initiation of exchange is completely epistatic to a mutation in RED1. Finally, mutations in either HOP1 or RED1 reduce the number of double-strand breaks observed at the HIS2 meiotic recombination hotspot.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1207519Documentos Relacionados
- Competition between Adjacent Meiotic Recombination Hotspots in the Yeast Saccharomyces Cerevisiae
- Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae.
- Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae
- Isolation of Mutants Defective in Early Steps of Meiotic Recombination in the Yeast Saccharomyces Cerevisiae
- Repair of specific base pair mismatches formed during meiotic recombination in the yeast Saccharomyces cerevisiae.