Analysis of Segmental Duplications and Genome Assembly in the Mouse
AUTOR(ES)
Bailey, Jeffrey A.
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
Limited comparative studies suggest that the human genome is particularly enriched for recent segmental duplications. The extent of segmental duplications in other mammalian genomes is unknown and confounded by methodological differences in genome assembly. Here, we present a detailed analysis of recent duplication content within the mouse genome using a whole-genome assembly comparison method and a novel assembly independent method, designed to take advantage of the reduced allelic variation of the C57BL/6J strain. We conservatively estimate that ∼57% of all highly identical segmental duplications (≥90%) were misassembled or collapsed within the working draft WGS assembly. The WGS approach often leaves duplications fragmented and unassigned to a chromosome when compared with the clone-ordered-based approach. Our preliminary analysis suggests that 1.7%–2.0% of the mouse genome is part of recent large segmental duplications (about half of what is observed for the human genome). We have constructed a mouse segmental duplication database to aid in the characterization of these regions and their integration into the final mouse genome assembly. This work suggests significant biological differences in the architecture of recent segmental duplications between human and mouse. In addition, our unique method provides the means for improving whole-genome shotgun sequence assembly of mouse and future mammalian genomes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=479105Documentos Relacionados
- Recent segmental and gene duplications in the mouse genome
- Genome-wide detection of segmental duplications and potential assembly errors in the human genome sequence
- Segmental Duplications: Organization and Impact Within the Current Human Genome Project Assembly
- Segmental Duplications and Copy-Number Variation in the Human Genome
- Recent Segmental Duplications in the Working Draft Assembly of the Brown Norway Rat