Analysis of sh-m6233, a mutation induced by the transposable element Ds in the sucrose synthase gene of Zea mays

AUTOR(ES)

Weck, E.

RESUMO

The unstable allele sh-m6233 caused by insertion of the transposable element Ds into the sucrose synthase gene of maize, was cloned. The mutation is caused by the insertion of an ˜4 kb DNA segment, consisting of two identical Ds elements of ˜2000 bp length, of which one is inserted into the center of the other in inverted orientation. This structure is, at the level of restriction mapping and partial DNA sequencing, identical to the double Ds element found in a larger insert in the mutant allele sh-m5933. 8 bp of host DNA are duplicated upon insertion. In a revertant, a 6-bp duplication is retained.

Documentos Relacionados

• Genomic clones of a wild-type allele and a transposable element-induced mutant allele of the sucrose synthase gene of Zea mays L
• The maize transposable element system Ac/Ds as a mutagen in Arabidopsis: identification of an albino mutation induced by Ds insertion.
• Structure of the sucrose synthase gene on chromosome 9 of Zea mays L
• Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays
• Transcription of transposable element Activator (Ac) of Zea mays L