Analysis of the Import of Carboxyl-Terminal Truncations of the 23-Kilodalton Subunit of the Oxygen-Evolving Complex Suggests That Its Structure Is an Important Determinant for Thylakoid Transport.

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RESUMO

A series of deletions from the carboxyl terminus of the 23-kD subunit of the photosynthetic oxygen-evolving complex OE23 revealed that these truncations result in various degrees of inhibition of translocation across thylakoid membranes and their subsequent assembly to the oxygen-evolving complex. Import of in vitro translated precursors across the chloroplast envelopes was not inhibited by these truncations. Time-course studies of the import of truncated OE23 precursors into intact chloroplasts revealed that the stromal intermediate was subsequently translocated into the thylakoid lumen, where it was processed to a smaller size and rapidly degraded. In contrast to the full-length OE23 intermediate, the truncated intermediate forms that accumulated in the stroma as a result of de-energization of thylakoid membranes could be found associated with the membrane rather than free in the stroma. Protease digestion experiments revealed that the deletions evidently altered the folded conformation of the protein. These results suggest that the carboxyl-terminal portion of the OE23 precursor is important for the maintenance of an optimal structure for import into thylakoids, implying that the efficient translocation of OE23 requires the protein to be correctly folded. In addition, the rapid degradation of the truncated forms of the processed OE23 within the lumen indicates that a protease (or proteases) active in the lumen can recognize and remove misfolded polypeptides.

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