Analysis of the Ribonucleic Acid of Murine Leukemia Virus
AUTOR(ES)
Bader, John P.
RESUMO
Cells producing the Rauscher strain of murine leukemia virus (MLV) were exposed to 3H-uridine, and labeled virus was collected at hourly intervals. Ribonucleic acid (RNA) extracted from virions (vRNA) had a characteristic single peak when analyzed by electrophoresis in polyacrylamide-agarose composite gels. Exposure of vRNA to dimethyl sulfoxide, urea, formaldehyde, or heat altered the mobility to a faster moving form (vRNA′). This vRNA′ sedimented more slowly than native vRNA in sucrose gradients. Incubation of labeled virions at 37 C resulted in fragmentation of viral RNA which was detectable only after denaturation. Also, large differences in the temperature required for the change from vRNA to vRNA′ were seen with alterations in NaCl concentration. These experiments demonstrate that the vRNA of MLV is held in a specific conformation by hydrogen bonds distributed over a large part of the molecule. The possibility that an undefined factor is associated with viral RNA is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=375894Documentos Relacionados
- Electron Microscopic Observations on the Ribonucleic Acid of Murine Leukemia Virus
- Characterization of Intracellular Ribonucleic Acid Specific for the Murine Sarcoma-Leukemia Virus Complex
- Ribonucleic Acid Components of Murine Sarcoma and Leukemia Viruses
- The nucleic acid of a murine leukemia virus.
- Mutational analysis of the envelope gene of Moloney murine leukemia virus.
