Analysis of tissue-specific methylation patterns of mouse mammary tumor virus DNA by two-dimensional Southern blotting.

AUTOR(ES)

Fanning, T G

RESUMO

We used a two-dimensional Southern blotting procedure to analyze the tissue-specific methylation patterns of the five endogenous mouse mammary tumor viruses in the GR/A mouse strain. Our findings suggest that in certain tissues (brain, kidney, and liver) all proviruses are extensively methylated. In other tissues (spleen, placenta, and testes) all proviruses are hypomethylated to some degree. In these tissues individual proviruses display both quantitative and qualitative differences in methylation. We interpret the general patterns of tissue-specific hypomethylation in terms of a "hitch-hiker" model: mouse mammary tumor virus proviral methylation patterns reflect the tissue-specific activity of neighboring sequences. The observation that certain sites on particular proviruses are differentially methylated in a tissue-specific fashion may reflect tissue-specific differences in the makeup or conformation, or both, of proviral-containing chromatin.

Documentos Relacionados

• Analysis of secondary modifications of mouse mammary tumor virus proteins by two-dimensional gel electrophoresis.
• The chromosomal integration site determines the tissue-specific methylation of mouse mammary tumour virus proviral genes.
• Negative regulation in correct tissue-specific expression of mouse mammary tumor virus in transgenic mice.
• Tissue and strain-specific patterns of endogenous proviral hypomethylation analyzed by two-dimensional gel electrophoresis.
• Analysis of two-dimensional protein patterns from mouse embryos with different trisomies.